cdna release solution (Complete Genomics Inc)

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Complete Genomics Inc cdna release solution
Cdna Release Solution, supplied by Complete Genomics Inc, used in various techniques. Bioz Stars score: 99/100, based on 464 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+release+solution/Stereo-seq+Transcriptomics+Set+for+FFPE/pmc12866786-258-50-53
Average 99 stars, based on 464 article reviews

cdna release solution - by Bioz Stars, 2026-09

99/100 stars

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Article Title: An invasive zone in human liver cancer identified by Stereo-seq promotes hepatocyte-tumor cell crosstalk, local immunosuppression and tumor progression.
Article Snippet: After in situ reverse transcription, tissue patches were washed twice with 0.1× SSC buffer and digested with tissue removal solution (STOmics, 1000028505) at 37 °C for 30min. .. The cDNA-containing chips were then incubated with 400 μL cDNA release solution (STOmics, 1000028512) for 3 h at 55 °C, and then washed once with 400 μL of 0.1× SSC buffer (Thermo Fisher Scientific, AM9770). .. All products were purified using 0.8× Ampure XP Beads (Vazyme Biotech, Nanjing, China), and were amplified with KAPA HiFi Hotstart Ready Mix (Roche, Basel, Switzerland) using 0.8 μM cDNA-PCR primers.

Article Title: SLPI⁺ AT2-Like Cells Orchestrate Lung Adenocarcinoma Invasion via Wnt Pathway Activation and Stromal Crosstalk in a Spatially Defined Margin Niche.
Article Snippet: RNA from the treated tissues was bound by the DNBs on the chips and converted to cDNA via reverse-transcribed at 42 °C for 1 h, utilizing a mixture of 10 U μL−1 reverse transcriptase, 2 U μL−1 RNase inhibitor, 1 mM dNTPs, 1 M betaine PCR additive, 7.5 mMMgCl2, 5 mM DTT, 2.5 μM Stereo-TSO, and 1× First-Strand buffer (BGI). .. The tissue was then rinsed twice with 0.1× SSC buffer, enzymatically cleared using tissue removal buffer (STOmics, 1 000 028 505) at 55 °C for 30 min, and rinsed twice more with 0.1× SSC buffer. cDNA was detached from the chips through overnight incubation at 55 °C in 3 mL cDNA release solution (STOmics, 1 000 028 512), succeeded by a single rinse with 3 mL nuclease-free water. .. The cDNA was refined using 0.8 × Ampure XP Beads (Vazyme Biotech, Nanjing, China) and amplified with KAPA HiFi Hotstart ReadyMix (Roche, Basel, Switzerland), 0.8 μM primers, and the thermal profile: 95 °C for 5 min; 15 cycles of 98 °C for 20 s, 58 °C for 20 s, and 72 °C for 3 min, followed by 72 °C for 5 min. Amplified products were refined with 0.6 × Ampure XP Beads, and their concentration wasmeasured using a Qubit dsDNA Assay Kit (Thermo Fisher Scientific, USA).

Article Title: An invasive zone in human liver cancer identified by Stereo-seq promotes hepatocyte–tumor cell crosstalk, local immunosuppression and tumor progression
Article Snippet: After in situ reverse transcription, tissue patches were washed twice with 0.1× SSC buffer and digested with tissue removal solution (STOmics, 1000028505) at 37 °C for 30 min. .. The cDNA-containing chips were then incubated with 400 μL cDNA release solution (STOmics, 1000028512) for 3 h at 55 °C, and then washed once with 400 μL of 0.1× SSC buffer (Thermo Fisher Scientific, AM9770). .. All products were purified using 0.8× Ampure XP Beads (Vazyme Biotech, Nanjing, China), and were amplified with KAPA HiFi Hotstart Ready Mix (Roche, Basel, Switzerland) using 0.8 μM cDNA-PCR primers.

Article Title: SLPI ⁺ AT2‐Like Cells Orchestrate Lung Adenocarcinoma Invasion via Wnt Pathway Activation and Stromal Crosstalk in a Spatially Defined Margin Niche
Article Snippet: RNA from the treated tissues was bound by the DNBs on the chips and converted to cDNA via reverse‐transcribed at 42 °C for 1 h, utilizing a mixture of 10 U μL −1 reverse transcriptase, 2 U μL −1 RNase inhibitor, 1 mM dNTPs, 1 M betaine PCR additive, 7.5 mM MgCl 2 , 5 mM DTT, 2.5 μM Stereo‐TSO, and 1× First‐Strand buffer (BGI). .. The tissue was then rinsed twice with 0.1× SSC buffer, enzymatically cleared using tissue removal buffer (STOmics, 1 000 028 505) at 55 °C for 30 min, and rinsed twice more with 0.1× SSC buffer. cDNA was detached from the chips through overnight incubation at 55 °C in 3 mL cDNA release solution (STOmics, 1 000 028 512), succeeded by a single rinse with 3 mL nuclease‐free water. .. The cDNA was refined using 0.8 × Ampure XP Beads (Vazyme Biotech, Nanjing, China) and amplified with KAPA HiFi Hotstart ReadyMix (Roche, Basel, Switzerland), 0.8 μM primers, and the thermal profile: 95 °C for 5 min; 15 cycles of 98 °C for 20 s, 58 °C for 20 s, and 72 °C for 3 min, followed by 72 °C for 5 min. Amplified products were refined with 0.6 × Ampure XP Beads, and their concentration was measured using a Qubit dsDNA Assay Kit (Thermo Fisher Scientific, USA).

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